The Role of Antiviral Action of a Novel Multifunctional 3D Nano-vaccine against HIV-1 and Multiple Drug Resistant Pharmacotherapy: Towards Nano-robotic Utilization. project time duration - 02 years and 06 months
According to the World Health Organization there were 35 million people living with HIV at the end of 2013. In the present study, 2-ketoglutaric acid and Aspartic acid multi-functionalized nanoparticles were developed as a nano-vaccine. Statistical analysis of student t test p value was less than 0.05. The method is 180 500 times less expensive, 24 times reduced toxicity, 74.8% more efficient and 15000 times consumer friendly when compared to the current antiretrovirals. Investigation in these interactions provides the foundation for possible future therapy for HIV-1 infection.
Using nano technology as an aniticancer agent; Effect of catechin funtionalized iron oxide nanoparticles on leukemic monocytes. project time duration - 02 years
Cancer is a major cause of mortality in most developed and underdeveloped countries of the world, and is now considered to be secondary only to that of myocardial infarction. It is a growing public health menace and more than six million new cases of cancer are reported every year. With the emergence of Nano technology, most of research groups are interested on targeted cancer therapies in order to minimize the side effects on healthy tissues while delivering the drug to pre-determined targets located in transformed cells. In this research the effects of catechin coated iron oxide nanoparticles were evaluated on in-vitro established cell line model; THP1 cells. A flavonoid compound that contains in green tea was developed as surface modified nanoparticles. Subsequently, its effect was evaluated. Results were analyzed using standard student T test by compared with the control. According to the results it was given a statistically significance value.
Anti Proliferative and Apoptotic Effects of Ellagic Acid Functionalyzed Iron Oxide Nano Particles on Endometrial Cancer (AN3CA) Cells project time duration - 02 years
Nanotechnology has integrated with bioscience to offer several remedial actions against cancer. In this study, the known cancer inhibitory action of the ellagic acid (EA) was tested on chemo – endocrine resistant endometrial cancer cells AN3CA by coating EA on to magnetic iron oxide nanoparticles (IONPs). Synthesis of IONPS were carried out by co-precipitation method using Fe2+ and Fe3+ salts in the presence of polyethylene glycol (PEG). EA coated IONPs (EA-IONPs) were then prepared by post coating treatment. Synthesized nanoparticles were characterized using FTIR and TEM. Anti-proliferative effects of pure EA, IONPs and EA-IONPs on AN3CA cells were evaluated by Sulphorodamine B assay and induction of apoptosis was tested by evaluating morphological changes. Pro apoptotic effects of EA-IONPs on AN3CA cells were further confirmed by (a) assessing Acridine Orange / Ethedium Bromide staining under the fluorescent microscopy, (b) evaluating levels of caspase 3/7 in treated AN3CA at 48 h post incubation. EA-IONPs exerted a significant dose dependent cytotoxicity to AN3CA as evident from SRB assay (IC50 = 6.6 μg/mL) while EA shows comparatively less cytotoxicity (IC50 = 24.7 μg/mL). Characteristic morphological changes associated with apoptosis in EA-IONPs treated AN3CA cells were observed using phase contrast and fluorescent microscopy. EA-IONPs significantly (P<0.05) increased the caspase-3/7 levels dose dependently and has increased the mRNA expression (P<0.05) of tumor suppressor gene p53. The EA-IONPs may therefore mediate its anti-cancer effect, through modulation of cytotoxicity and apoptosis via, p53, caspase 3/ 7 mediated pathways in AN3CA. Based on overall results EA-IONPs exhibit an improved capacity to inhibit the growth of cancer cells.
“HEALTH PAL” – AN EFFECTIVE HEALTH MONITORING APP project time duration - one year
In this society where elderly people mostly live alone, many people suffering from heath conditions such as Heart Diseases fail to identify their conditions at the critical time before they have an attack. Especially if it is an unconscious attack the individual would be helpless. To combat this issue I have developed an android application that works in- synchronization with a pulse-oximeter to early detect such critical health conditions and automatically alert emergency services. This application was tested on a focus group of 30 individuals with no history of heart diseases as the control group and a group of 30 individuals with a history of heart diseases as the experimental group. A group of 12 surgical patients were also monitored during surgical procedures with my application and it automatically activated an emergency alert when the patients’ vitals were at a critical level. Since the clinical trial was a success my app can be used by people prone cardiac diseases living in isolated environments to identify attacks and ultimately save lives.
Effect of Artocarpus heterophyllus and Cinnamomum verum extract to increase platelet count in wistar rats. project time duration - one year
Over 50,000 patients were suffering from dengue fever and 205 deaths were reported during the year 2011 and 2012 in Sri Lanka. Dengue virus induces severe thrombocytopenia. People consume Ayurvedic medicine together with allopathic medicine in order to increase their platelet counts. Initial survey carried out in National Hospital and Lady Ridgway Hospital of Sri Lanka revealed that people consume boiled extracts of Cinnamomum verum and Artocarpus heterophyllus when there is thrombocytopenia due to dengue fever. Research was conducted to investigate the efficacy of plant extracts to increase the platelet count. A. Results were analysed using standard student T test. Rats ingested aqueous extract of Cinnamomum verum and Artocarpus heterophyllus showed significantly high platelet count compared to control. Significance was (p=0.003513) and (p =0.030618) respectively. Both extracts has the ability to increase chemically induced thrombocytopenia in in-vivo models.
Novel way of irrigation water conservation - A simplified and low costing field capacity testing device to make the soil and plants healthy & wealthy. project time duration - 06 months
This project was carried out in order to investigate a modest method of testing field capacity. The experiment was based on creating a device which raise combination with the experimental test of field capacity with novel steps. The results of the experiment were tested by fixing the created device in three different soil conditions. The experiment was done under three sub experiments.
Although there are modern ways of testing moisture but there aren’t any way to test the field capacity at the same moment of working in the field. The creation of this device was based on simplicity, low cost, understandable scientific technology and following easy steps to receive the proper indication in return to limit the over exploitation of water to make the farming process and water process effectively. If this process is performing well it will be an easy task to conserve the exploitation of water during watering while creeping a healthy harvest by keeping the soil in a compliment water percentage and delivering significant environmental benefits in local and global scenario.
Textile used, extracted formaldehyde base mediums as preservatives for laboratory specimens. project time duration - 08 months
Formaldehyde is a wide using chemical as a resin or pigment in textile industry for the finishing process. At the same point formaldehyde release through textile waste water is in a high stage. Due to the high expose, considering the effect on human body by formaldehyde, Irritation and allergic contact dermatitis have been associated with exposure of the skin to formaldehyde at levels higher than those encountered in drinking-water. Furthermore high concentration is a great threat to the biological species depends on water sources. Formaldehyde has been the commonly using product for laboratory specimen preservation as 10% formal saline. In this study textile used formaldehyde extracted from waste water by using alcohol based fractional distillation was used in an agar medium and treats with X nano particles were experiment as alternatives to 10% formal saline. Freshly taken samples, human spleen was preserved under the 10% formal saline medium as the control, After 72 hours of preservation period Antimicrobial effect of the preservative medium and Histopathalogical test to evaluate the specimen preservation status was done. Concentration of formaldehyde of waste water sample, before and after the fractional distillation was measured by reacting formaldehyde with brady reagent. According to the study it can be speculate that newly developed medium can be reused after treated with X nano particles to the preservation process and it will help to minimize the emission of formaldehyde through water to water resources and to minimize the usage of fresh formaldehyde per year for preservation process.
Drosophila melanogaster Repellent Ultra sonic device project time duration - 06 months
In the world today people have taken different measures to prevent insects from causing harm to their crop cultivations. But those existing methods have major and serious drawbacks and causes imbalance in the bio diversity. Most of those techniques involve using Agro chemicals in the industry. But these agro chemicals gets absorbed into the plants and then get accumulated on the human body when these plants are taken as food. And most of these chemicals are carcinogenic. Therefore we came up with an alternative method to repel harmful insects. Our idea is to implement a device with ultra-sonic emitters that will repel the insects. This device we are planning to implement will be a low cost , efficient device. This device was planned to be implemented to surpass the major drawbacks shown by the agro chemical usage. The device was able to show a good repellence in the case of Drosophila melanogaster in a wide range of ultra sound frequencies. As every organism has to be there in the environment to maintain the dynamic nature of bio diversity the 20% un repelled fruit flies are not taken into account. Because our major task was to repel the fruit flies which were of commercially harmful to the crops in large scale. According to the literature survey conducted we didn’t find any ultra sonic fruit fly repellent device, so we are focusing to increase the range of the device and the strength of the interface. The ultra sonic sound which was the basis of this experiment can be concluded as a better way of fruit fly repellence as the bio chemical hazards are minimized meanwhile showing a successful percentage of 80% fruit fly repellence.
A Capable Desalination Unit to Clean Hard & Fluoridated Water To Make Healthy Kidney” project time duration - 06 months
Water is a gift that donates to the living people from nature. The value of this water is becoming more and higher because the clean drinking water no more in the world. And we must remember that “there is no clean water there is no more life” in the earth as human beings. There’re large amount of ground water in Sri Lanka, but some areas that water is not suitable for drinking because it can be caused Kidney Diseases which are commonly observed in many parts of Dry Zone of Sri Lanka, mostly in North Central Province. Chronic Renal Failure [CRF] has recently shown a marked increase in some areas in the Anuradhapura and Polonnaruwa districts. Approximately 72% of the population of Sri Lanka lives in a rural environment with a close association with the immediate physical environment obtaining their drinking water directly from the ground. The ground water of these areas is generally of the Ca/Mg with CO3– / (HCO3)2 / SO4 type which are named as hard water. And that water is content with Fluoride (F–) also. So we selected Anuradhapura and Polonnaruwa districts as our project. Our project introducing a capable desalination unit that can desalinate this Hard and Fluoridated water to purify water that is suitable for drinking or any other uses. In the process of making our machine, first we though about a scientific method. Last we agreed to use the Boil’s Law. So we use a manual vacuum unit, a bi convex lens and a special shape glass box to make our desalination unit. Then we desalinate that Hard and Fluoridated water using the sun rays. After that we tested pH, Electrical Conductivity, Fluoride and he Titration in desalinated water. From that we saw a large desalinating process happen in the water. During the time of making our machine we calculate the pH, Electrical conductivity, Fluoride and the Titration and we got the average measures from five test terms. From those calculations of Hard and Fluoridated water and the desalinated water we can get an idea about the reduced amount of minerals and we can say our machine is ready to use to the process of desalination of Hard and Fluoridated water in North Central Province of Sri Lanka.
Adaption of a fungal in debased water samples for bio sorption of heavy metals project time duration - 06 months
The main threats to human health from heavy metals are associated with exposure to lead, cadmium, chromium etc. These metals have been extensively studied and their effects on human health regularly reviewed by international bodies such as the WHO. Heavy metals have been used by humans for thousands of years. Although several adverse health effects of heavy metals have been known for a long time, exposure to heavy metals continues, and is even increasing in some parts of the world, in particular in less developed countries, though emissions have declined in most developed countries over the last 100 years. Bio sorption or adsorbing biomass has been a potent way which can remove harmful metals like lead, cadmium, cobalt, chromium etc. from water bodies. In this study Pennisillium species, a fungi inoculated by lemon, a citric acid fruit was used to test the biosorption of heavy metals in different water samples. The results was significant and it was speculated that penisiilum species was in a active stage of bio sorption heavy metals from water bodies
STUDY ON DEVIATION OF ANNUAL RAINFALL PATTERN IN COLOMBO DISTRICT project time duration - one year
Rainfall is one of the influencing factors for human activities which is defined as the amount of water falling in rain within a given time and area, usually expressed as a hypothetical depth of coverage. The impact of rainfall is not limited to agricultural based regions but also to industrialized urban areas. The rainfall received in any region can vary with physical factors as well as human interactions. The physical factors that can cause such variation could include micro-climatic phenomenon or global changes such as photochemical smog and global warming. The results obtained through observation by the GLOBE SOCIETY of our school clearly indicates that there are significant changes in the rainfall pattern of Colombo district which belongs to the wet zone of Sri Lanka. Annually Sri Lanka receives different rainfall types, which are Convectional rainfall, Monsoonal rainfall and Expressional rainfall. As a result of abrupt changes in nature the rainfall patterns of Colombo has been affected and this study focuses on these deviations and the changes that may occur on the crop calendar due to this. The cultivation in Sri Lanka is carried out in the two main seasons ‘Yala’ and ‘Maha’. According to the typical rainfall patterns and the crop calendar the highest rainfall in Colombo (wet zone) is expected in the months May and October. Yet our study concludes that these patterns have changed over the past decade (2005 – 2015). Through this study we are focusing on informing the general public and the cultivators about the deviation in typical rainfall pattern in Colombo district.
OBSTACLE DETECTING ULTRA SONIC DEVICE FOR BLIND PEOPLE project time duration - one year
In the world today people have taken different measures with the intention of helping the blind society. But as those attempts don’t seem to be covering all the areas regarding blind people we also paid attention in regarding this matter. So as our hypothesis we came up with an idea of implementing a device which uses ultra-sonic sensors to identify obstacles which can harm blind people when walking. Unlike the blind stick this device will be much convenient and reliable. This device uses ultrasonic sound waves to detect obstacles in the path way of the blind person. It gives off a sound when it detects an obstacle. The sound will be high frequent when the obstacle is closer to the device and gives a low frequent sound when the obstacle is far away. In the procedure of creating the device we programmed a Lakduino which is a compatible version of the Arduino UNO to carry out the tasks. In order to detect obstacles we used an ultra-sonic sensor called “HC-SR04”. Then we used a buzzer to give off a sound. We tested the device in 2 ways a) Range and capability of the device b) Practicality of the Device. So according to our results the device was successful. Since this device is still in the prototype stage we will look forward in developing this device. So as our conclusion we’ve planned to add an array of ultrasonic sensors to detect obstacles in the ground level.
Let’s make factory emitted water eco friendly project time duration - 06 months
Water is a gift that donates to the living people from nature. The value of this water is becoming more and higher because nowadays water is being polluted by the humans. Because of these few undestroyed water is left in the world for the usage of man and animals. In the future this small amount of water can be destroyed and drinking water will be vanished from earth. For this reason we must have to protect water. Factories are a main polluter of water. From them large amount of polluted water is released to the water sources. This polluted water contains carbon, different kinds of chemicals, oils and methane as a byproduct. These chemicals and other things reduce the oxygen limit in the water and it’s a threat to the aquatic plants and animals in that water. Because of them different kinds of mosquitoes are grown and it cause to the different kinds of diseases like Dengue and Malaria. There for the factories have to take an action to release their waste water favorable to the Our project introducing a filter machine made out of natural materials that can filter these polluted water to purify water that is suitable for aquatic life and to reduce the methane percentage which is cause to the climate changes. We selected Orugodawatte Canal as our project site. In there we saw a small tunnel that connected to the canal from a car yard and to a leather. In the process of making our machine, first we selected some natural materials like coconut shells, fibers, straw, ipil ipil, charcoaled rice husk, tea waste, and different kinds of gravels like ciolite, sand and ingini eta. Then we filtered polluted water using them separately and tested pH, Turbidity in filtered water. From that we saw a large filtering process happen in the filtered water through the materials Charcoaled rice husk, Igini eta, Ipil ipil, Sand and ciolite. So we selected these materials to make our filter. During the time of making our machine we calculate the pH, Turbidity, Temperature, Chemical oxygen demand and Biochemical Oxygen Demand and we got the average measures from five water test terms. From those two calculations of polluted water and the filtered water we can get an idea of the amount of reducing chemicals and we can say that our machine is ready to use to the process of filtration of factory emitted water.